Ancillary Testing in Cytology

Ancillary Testing

Course Description

This course will introduce the learner to companion technologies utilized by today’s cytology professional. Highlights include course material on molecular diagnostics, immunohistochemistry, and digital image analysis.

Requirements

This module requires that you sign up for a free account with the Cytology Education Learning Lab (C.E.L.L.)

Learning Outcomes

1. The learner will define and explain the theory, principles, and indications for tests in cytology/pathology including:

a) Flow cytometry
b) Molecular Diagnostics (e.g., Polymerase chain reaction (PCR), Next-Gen Sequencing (NGS), microarrays)
c) In situ hybridization (e.g., FISH, CISH, ISH)
d) Immunochemistry (e.g., IHC/ICC)
e) Special stains (e.g., AFB, GMS, Fite, mucicarmine, Congo red)
f) Digital image-analysis (e.g., Quantitative IHC/ICC)
g) Electron microscopy

2. The learner will evaluate and analyze the findings and clinical significance of results in cytology/pathology for:

a) Flow cytometry
b) Molecular Diagnostics (e.g., Polymerase chain reaction (PCR), Next-Gen Sequencing (NGS), microarrays)
c) In situ hybridization (e.g., FISH, CISH, ISH)
d) Immunochemistry (e.g., IHC/ICC)
e) Special stains (e.g., AFB, GMS, Fite, mucicarmine, Congo red)
f) Digital image-analysis (e.g., Quantitative IHC/ICC)

3. The learner will define and explain the theory, principles, and indications for techniques in cytology/pathology including:

a) Telepathology/telecytology
b) Digital image acquisition, management (e.g., storage, retrieval, and sharing pathology information)
c) Image capture via static photography, dynamic telepathology (e.g., viewing real-time images and virtual slides/whole-slide imaging)
d) Quantification of specific image features (e.g., DNA analysis, morphometric analysis, FISH)
e) Web conferencing
f) Educational presentations

4. The learner will utilize clinical digital cytology/pathology as part of a diagnostic team including, but not limited to, rapid on-site evaluation, frozen section, and second opinion consultation.

5. The learner will evaluate cases (slides and/or tissue blocks) to select samples that contain adequate morphologic material for companion testing.